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مایع سلومیکی

Dear sir/Madam
We are writing with regard to give you the first report of antimicrobial assay of the Selomic compound
In this part, two bacterial strains including Escherichia coli (DSM 498) and Staphylococcus aureus (ATCC 5923) were recruited. Brain Heart Infusion agar plates were used for cultivation of bacteria Plates were incubated at 37°C for 24 hr and used for antimicrobial tests. Antimicrobial assay was performed by disk diffusion method. Bacterial suspensions with the turbidity of 0.5 MacFarland standards (1.5×108 cell /mL) were prepared. Mueller-hinton agar plates were surface inoculated with 100 µL of bacterial suspension. Sterile blank disks were deposited on the surface of inoculated plates. A 10-20-40 and 80 µL of Selomic was introduced into blank disks. Plates were incubated at 37°C and examined after 24 hr
 The results show that no bacteria were grown in the presence of selomic in all the plates. In other word, all the four inoculated selomic inhibited growth of E.coli and S.aerous
At the next step, 4mL of brain heart infusion broth and 1mL of bacterial suspensions with the turbidity of 0.5 MacFarland standard were mixed. Then 100 µL of Selomic was added and incubated at 37°C.  After 24 hr, there were seen no turbidiry in the test tubes indicating inhibition the growth of bacteria
Further study should be done to determine MIC and MBC of Selomic against E.coli and S.aureus
With best regards
 
 
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